A Radioimmunoassay Method for Simultaneous Determination of Pregnenolone, Pregnenolone Sulfate, Dehydroepiandrosterone and Dehydroepiandrosterone Sulfate in Human Plasma
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چکیده
منابع مشابه
Serum dehydroepiandrosterone, dehydroepiandrosterone sulfate, and pregnenolone sulfate concentrations in patients with hyperthyroidism and hypothyroidism.
BACKGROUND Dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEA-S) have been suggested to have protective effects against cardiovascular disease, cancer, immune-modulated diseases, and aging. We examined serum concentrations of DHEA, DHEA-S, and pregnenolone sulfate (PREG-S) in patients with thyroid dysfunction. METHODS Steroids extracted with methanol from serum sample were...
متن کاملGonadotropin-releasing hormone stimulates the biosynthesis of pregnenolone sulfate and dehydroepiandrosterone sulfate in the hypothalamus.
The sulfated neurosteroids pregnenolone sulfate (Δ(5)PS) and dehydroepiandrosterone sulfate (DHEAS) are known to play a role in the control of reproductive behavior. In the frog Pelophylax ridibundus, the enzyme hydroxysteroid sulfotransferase (HST), responsible for the biosynthesis of Δ(5)PS and DHEAS, is expressed in the magnocellular nucleus and the anterior preoptic area, two hypothalamic r...
متن کاملThe neurosteroids dehydroepiandrosterone sulfate and pregnenolone sulfate inhibit the UNC-49 GABA receptor through a common set of residues.
Neurosteroids are endogenous neuromodulators that bind and allosterically regulate GABA(A) receptors. Residues were recently identified in the first transmembrane domain (M1) of GABA(A) receptor subunits that are important for neurosteroid modulation. We are studying the inhibition of GABA(A) receptors by sulfated neurosteroids. One of these neurosteroid, pregnenolone sulfate (PS), depends on s...
متن کاملMetabolism of dehydroepiandrosterone sulfate and estrone-sulfate by human platelets.
The aim of the present research was to study the uptake of DHEAS, and to establish the intracrine capacity of human platelets to produce sex steroid hormones. The DHEAS transport was evaluated through the uptake of [(3)H]-DHEAS in the presence or absence of different substrates through the organic anion transporting polypeptide (OATP) family. The activity of sulfatase enzyme was evaluated, and ...
متن کاملPregnenolone and pregnenolone sulfate metabolism by human fetal testes in vitro.
}lomogenates of testes from human fetuses of 12-16 weeks gestation were incubated in vitro with pregnenolone-4-14C and pregnenolone-7-3Hsulfate. Dehydroepiandrosterone sulfate and pregnenediol sulfate, each bearin~ the tritium label only, were identified in the conjugated fraction. Progesterone, 17~-hydroxyprogesterone, 17a-hydroxypregnenolone, dehydroepiandrosterone, androstenedione and testos...
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ژورنال
عنوان ژورنال: Folia Endocrinologica Japonica
سال: 1976
ISSN: 0029-0661
DOI: 10.1507/endocrine1927.52.10_1008